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Glossary

Meta-analysis: pooling separate studies into one weighted estimate

A meta-analysis statistically combines the results of separate studies measuring the same effect, weighting each by its precision, to produce a pooled estimate with a confidence interval. It usually sits inside a systematic review, and it is only as sound as the studies pooled — combining biased trials yields a precise wrong answer.

Why it matters when you are writing

The output most readers recognize is the forest plot: one row per study showing its effect and interval, with a diamond at the bottom for the pooled estimate. Larger and more precise studies pull the diamond further, which is the point of weighting. Reading the plot rather than the summary sentence tells you whether the pooled figure represents a consistent picture or averages across studies that disagree.

Heterogeneity is the question that decides whether pooling was legitimate at all. Statistics such as I² quantify how much variation exceeds chance, but the substantive judgment comes first: do these studies measure the same thing in comparable populations? Pooling a school-based intervention with a clinical one produces a number that describes nothing real, however tidy the confidence interval looks.

Publication bias distorts the input. Studies that find nothing are published less often and later, so the literature available to pool is skewed toward positive results. Funnel plots and tests for asymmetry give a partial check, and searching trial registries and gray literature gives a better one. Neither fully solves it, which is why a careful meta-analysis discusses the risk rather than declaring it absent.

IN PRACTICE

What a pooled estimate reports

Twelve trials, 3,480 participants: pooled standardized mean difference 0.28, 95% CI [0.11, 0.45], I² = 62%. The interval excludes zero; the heterogeneity means the pooled figure describes a range of study contexts, not a single reliable effect.

OFTEN CONFUSED WITH

What Meta-analysis is not

Systematic review
A systematic review is the whole protocol-driven process of finding and appraising studies; a meta-analysis is the statistical pooling step inside it.
Pooled analysis
A pooled analysis combines the raw individual-participant data from several studies; a meta-analysis combines their published summary results.

Questions about meta-analysis

How many studies does a meta-analysis need?

There is no fixed minimum, and pooling two studies is technically possible. With very few studies the estimate is unstable and heterogeneity cannot be assessed meaningfully.

What does I² tell me?

The proportion of variation across studies beyond what chance would produce. High values signal that the studies may not be measuring a common effect, so the pooled estimate needs cautious interpretation.

Can a meta-analysis be wrong?

Yes. It inherits every bias in the studies it pools, and adds selection bias if the search missed unpublished null results. Precision is not the same as accuracy.

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